anti egr 1 antibody (Cell Signaling Technology Inc)
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Anti Egr 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+egr+1/EGR1+Rabbit+mAb/pmc12215247-247-15-20
Average 96 stars, based on 333 article reviews
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other:Article Title: Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum Article Snippet: After washing with PBT at room temperature, secondary anti-rabbit or anti-mouse IgG antibodies conjugated with Alexa Fluor 488 or 546 (1:1000, TFS, #TA11008 or #A21123) were added and incubated at room temperature for 1 h. Samples were washed with PBS and mounted in Vectashield mounting medium with DAPI (Vector Laboratories Inc., #H-1200). Incubation:Article Title: Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles Article Snippet: Briefly, sections were rinsed in 0.1 M PBS 4 times for a total 20 min (each rinsing was kept at the rate of 4 times for a total of 20 min below) and then treated with 1% NaBH4 in 0.1 M PBS for 10 min. After rinsing in 0.1 M PBS, sections were incubated in 0.3% H2O2 in 0.1 M PBS for 20 min. After rinsing in 0.1 M PBS, sections were then incubated in 10% normal goat serum (NGS, Sigma-Aldrich, St. Luis, MO) in 0.3% Triton X-100 in 0.1 M PBS (TPBS) for 1 h at room temperature. .. Sections were then incubated in Article Title: Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum Article Snippet: After washing, samples were blocked with blocking buffer (3% BSA, Millipore-Sigma #A9647, and 5% normal goat serum, Cell Signaling Technologies (CST), #5425) in PBS) in a humidified chamber for 1 hat room temperature. .. The primary antibodies: rabbit anti-Jun (1:200, CST, #9165), Article Title: The ventromedial hypothalamic circuitry and male alloparental behaviour in a socially monogamous rodent species. Article Snippet: As prairie voles (Microtus ochrogaster) display spontaneous bi-parental care, and the ventromedial hypothalamus (VMH) has been implicated in reproductive behavior, we conducted experiments to test the hypothesis that the VMH neurochemical circuitry is involved in alloparental behaviors in male prairie voles.. We compared alloparental behaviors of adult, sexually-naïve male and female voles – both displayed licking/grooming, huddling, and retrieving behaviors toward conspecific pups.. We also stained for the immediate-early gene encoded early growth protein Egr-1 in the vole brain. Next-Generation Sequencing:Article Title: Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles Article Snippet: Briefly, sections were rinsed in 0.1 M PBS 4 times for a total 20 min (each rinsing was kept at the rate of 4 times for a total of 20 min below) and then treated with 1% NaBH4 in 0.1 M PBS for 10 min. After rinsing in 0.1 M PBS, sections were incubated in 0.3% H2O2 in 0.1 M PBS for 20 min. After rinsing in 0.1 M PBS, sections were then incubated in 10% normal goat serum (NGS, Sigma-Aldrich, St. Luis, MO) in 0.3% Triton X-100 in 0.1 M PBS (TPBS) for 1 h at room temperature. .. Sections were then incubated in Article Title: The ventromedial hypothalamic circuitry and male alloparental behaviour in a socially monogamous rodent species. Article Snippet: As prairie voles (Microtus ochrogaster) display spontaneous bi-parental care, and the ventromedial hypothalamus (VMH) has been implicated in reproductive behavior, we conducted experiments to test the hypothesis that the VMH neurochemical circuitry is involved in alloparental behaviors in male prairie voles.. We compared alloparental behaviors of adult, sexually-naïve male and female voles – both displayed licking/grooming, huddling, and retrieving behaviors toward conspecific pups.. We also stained for the immediate-early gene encoded early growth protein Egr-1 in the vole brain. Expressing:Article Title: Egr-1 increases angiogenesis in cartilage via binding Netrin-1 receptor DCC promoter Article Snippet: The homogenates (20 μg of protein) were separated by 8% SDS-PAGE and blotted on polyvinylidene fluori (PVDF) membranes (Bio–Rad Laboratories). .. Transblots were probed with the |
