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anti egr 1 antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti egr 1 antibody
    Anti Egr 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+egr+1/EGR1+Rabbit+mAb/pmc12215247-247-15-20
    Average 96 stars, based on 333 article reviews
    anti egr 1 antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum
    Article Snippet: After washing with PBT at room temperature, secondary anti-rabbit or anti-mouse IgG antibodies conjugated with Alexa Fluor 488 or 546 (1:1000, TFS, #TA11008 or #A21123) were added and incubated at room temperature for 1 h. Samples were washed with PBS and mounted in Vectashield mounting medium with DAPI (Vector Laboratories Inc., #H-1200).

    Incubation:

    Article Title: Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles
    Article Snippet: Briefly, sections were rinsed in 0.1 M PBS 4 times for a total 20 min (each rinsing was kept at the rate of 4 times for a total of 20 min below) and then treated with 1% NaBH4 in 0.1 M PBS for 10 min. After rinsing in 0.1 M PBS, sections were incubated in 0.3% H2O2 in 0.1 M PBS for 20 min. After rinsing in 0.1 M PBS, sections were then incubated in 10% normal goat serum (NGS, Sigma-Aldrich, St. Luis, MO) in 0.3% Triton X-100 in 0.1 M PBS (TPBS) for 1 h at room temperature. .. Sections were then incubated in rabbit anti-Egr-1 (1:3 K, Cell Signaling Technology, Inc., Danvers, MA) or rabbit anti-Iba-1 (1:10 K, Wako Chemicals) in 0.3% TPBS with 2% NGS at 4 °C for 2 nights. ..

    Article Title: Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum
    Article Snippet: After washing, samples were blocked with blocking buffer (3% BSA, Millipore-Sigma #A9647, and 5% normal goat serum, Cell Signaling Technologies (CST), #5425) in PBS) in a humidified chamber for 1 hat room temperature. .. The primary antibodies: rabbit anti-Jun (1:200, CST, #9165), rabbit anti-Egr-1 (1:200, CST, #4153), and mouse anti-Insulin (1:1000, Millipore-Sigma, #I-2018) were incubated overnight at 4 °C. .. Images were obtained using a Lionheart FX automated cell imaging microscope (Agilent-BioTek).

    Article Title: The ventromedial hypothalamic circuitry and male alloparental behaviour in a socially monogamous rodent species.
    Article Snippet: As prairie voles (Microtus ochrogaster) display spontaneous bi-parental care, and the ventromedial hypothalamus (VMH) has been implicated in reproductive behavior, we conducted experiments to test the hypothesis that the VMH neurochemical circuitry is involved in alloparental behaviors in male prairie voles.. We compared alloparental behaviors of adult, sexually-naïve male and female voles – both displayed licking/grooming, huddling, and retrieving behaviors toward conspecific pups.. We also stained for the immediate-early gene encoded early growth protein Egr-1 in the vole brain.

    Next-Generation Sequencing:

    Article Title: Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles
    Article Snippet: Briefly, sections were rinsed in 0.1 M PBS 4 times for a total 20 min (each rinsing was kept at the rate of 4 times for a total of 20 min below) and then treated with 1% NaBH4 in 0.1 M PBS for 10 min. After rinsing in 0.1 M PBS, sections were incubated in 0.3% H2O2 in 0.1 M PBS for 20 min. After rinsing in 0.1 M PBS, sections were then incubated in 10% normal goat serum (NGS, Sigma-Aldrich, St. Luis, MO) in 0.3% Triton X-100 in 0.1 M PBS (TPBS) for 1 h at room temperature. .. Sections were then incubated in rabbit anti-Egr-1 (1:3 K, Cell Signaling Technology, Inc., Danvers, MA) or rabbit anti-Iba-1 (1:10 K, Wako Chemicals) in 0.3% TPBS with 2% NGS at 4 °C for 2 nights. ..

    Article Title: The ventromedial hypothalamic circuitry and male alloparental behaviour in a socially monogamous rodent species.
    Article Snippet: As prairie voles (Microtus ochrogaster) display spontaneous bi-parental care, and the ventromedial hypothalamus (VMH) has been implicated in reproductive behavior, we conducted experiments to test the hypothesis that the VMH neurochemical circuitry is involved in alloparental behaviors in male prairie voles.. We compared alloparental behaviors of adult, sexually-naïve male and female voles – both displayed licking/grooming, huddling, and retrieving behaviors toward conspecific pups.. We also stained for the immediate-early gene encoded early growth protein Egr-1 in the vole brain.

    Expressing:

    Article Title: Egr-1 increases angiogenesis in cartilage via binding Netrin-1 receptor DCC promoter
    Article Snippet: The homogenates (20 μg of protein) were separated by 8% SDS-PAGE and blotted on polyvinylidene fluori (PVDF) membranes (Bio–Rad Laboratories). .. Transblots were probed with the rabbit anti-Egr-1 (Cell Signaling technology Inc., 1:1000), rabbit anti-VEGF antibody (Santa Cruz Biotechnology, 1:400), rabbit anti-DCC antibody (Santa Cruz Biotechnology, 1:400), or rabbit anti-Netrin-1 antibody (Santa Cruz Biotechnology, 1:400) to examine the protein expression in the lysates. .. The amount of protein transferred onto the membranes was verified by immunoblotting for GAPDH (Santa Cruz Biotechnology, 1:500).



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    Average density of <t>zif268-IR</t> cells ± standard error of the mean in the (A) medial dorsal striatum, (B) lateral dorsal striatum, (C) nucleus accumbens shell, and (D) nucleus accumbens core. Zif268 cell density was the lowest in primiparous hAPOEε4 rats compared to all other groups in all subregions of the dorsal striatum and nucleus accumbens. Average density of zif268-IR cells ± standard error of the mean in the (E) prelimbic cortex, (F) infralimbic cortex, and (G) anterior cingulate cortex. Across all subregions of the frontal cortex, zif268 cell density was lower in primiparous hAPOEε4 rats compared to primiparous wildtype rats and in hAPOEε4 rats compared to wildtype rats. Zif268 cell density was greater in the ACC compared to PrL and IL. Average density of zif268-IR cells ± standard error of the mean in the (H) granular retrosplenial cortex C and (I) granular retrosplenial cortex AB. Zif268 cell density was the lower in primiparous hAPOEε4 rats compared to primiparous wildtype rats in the granular retrosplenial cortex. Average density of zif268-IR cells ± standard error of the mean in the (J) dorsal CA1, (K) dorsal DG, and (L) ventral DG. Zif268 cell density in the dorsal CA1 was lower in primiparous rats compared to nulliparous rats. Of all hippocampal subregions, zif2668 cell density was lowest in the dentate gyrus, with lower density in the dDG compared to vDG. * indicates p <0.05. CA – cornu ammonis, DG – dentate gyrus, zif268 – zinc finger-containing transcription factor 268, IR – immunoreactive, hAPOEε4 – humanized APOEε4
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    Average density of <t>zif268-IR</t> cells ± standard error of the mean in the (A) medial dorsal striatum, (B) lateral dorsal striatum, (C) nucleus accumbens shell, and (D) nucleus accumbens core. Zif268 cell density was the lowest in primiparous hAPOEε4 rats compared to all other groups in all subregions of the dorsal striatum and nucleus accumbens. Average density of zif268-IR cells ± standard error of the mean in the (E) prelimbic cortex, (F) infralimbic cortex, and (G) anterior cingulate cortex. Across all subregions of the frontal cortex, zif268 cell density was lower in primiparous hAPOEε4 rats compared to primiparous wildtype rats and in hAPOEε4 rats compared to wildtype rats. Zif268 cell density was greater in the ACC compared to PrL and IL. Average density of zif268-IR cells ± standard error of the mean in the (H) granular retrosplenial cortex C and (I) granular retrosplenial cortex AB. Zif268 cell density was the lower in primiparous hAPOEε4 rats compared to primiparous wildtype rats in the granular retrosplenial cortex. Average density of zif268-IR cells ± standard error of the mean in the (J) dorsal CA1, (K) dorsal DG, and (L) ventral DG. Zif268 cell density in the dorsal CA1 was lower in primiparous rats compared to nulliparous rats. Of all hippocampal subregions, zif2668 cell density was lowest in the dentate gyrus, with lower density in the dDG compared to vDG. * indicates p <0.05. CA – cornu ammonis, DG – dentate gyrus, zif268 – zinc finger-containing transcription factor 268, IR – immunoreactive, hAPOEε4 – humanized APOEε4
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    Image Search Results


    Average density of zif268-IR cells ± standard error of the mean in the (A) medial dorsal striatum, (B) lateral dorsal striatum, (C) nucleus accumbens shell, and (D) nucleus accumbens core. Zif268 cell density was the lowest in primiparous hAPOEε4 rats compared to all other groups in all subregions of the dorsal striatum and nucleus accumbens. Average density of zif268-IR cells ± standard error of the mean in the (E) prelimbic cortex, (F) infralimbic cortex, and (G) anterior cingulate cortex. Across all subregions of the frontal cortex, zif268 cell density was lower in primiparous hAPOEε4 rats compared to primiparous wildtype rats and in hAPOEε4 rats compared to wildtype rats. Zif268 cell density was greater in the ACC compared to PrL and IL. Average density of zif268-IR cells ± standard error of the mean in the (H) granular retrosplenial cortex C and (I) granular retrosplenial cortex AB. Zif268 cell density was the lower in primiparous hAPOEε4 rats compared to primiparous wildtype rats in the granular retrosplenial cortex. Average density of zif268-IR cells ± standard error of the mean in the (J) dorsal CA1, (K) dorsal DG, and (L) ventral DG. Zif268 cell density in the dorsal CA1 was lower in primiparous rats compared to nulliparous rats. Of all hippocampal subregions, zif2668 cell density was lowest in the dentate gyrus, with lower density in the dDG compared to vDG. * indicates p <0.05. CA – cornu ammonis, DG – dentate gyrus, zif268 – zinc finger-containing transcription factor 268, IR – immunoreactive, hAPOEε4 – humanized APOEε4

    Journal: bioRxiv

    Article Title: Parity and APOEε4 genotype contribute distinct changes to functional connectivity across the middle-aged brain

    doi: 10.1101/2025.03.01.640999

    Figure Lengend Snippet: Average density of zif268-IR cells ± standard error of the mean in the (A) medial dorsal striatum, (B) lateral dorsal striatum, (C) nucleus accumbens shell, and (D) nucleus accumbens core. Zif268 cell density was the lowest in primiparous hAPOEε4 rats compared to all other groups in all subregions of the dorsal striatum and nucleus accumbens. Average density of zif268-IR cells ± standard error of the mean in the (E) prelimbic cortex, (F) infralimbic cortex, and (G) anterior cingulate cortex. Across all subregions of the frontal cortex, zif268 cell density was lower in primiparous hAPOEε4 rats compared to primiparous wildtype rats and in hAPOEε4 rats compared to wildtype rats. Zif268 cell density was greater in the ACC compared to PrL and IL. Average density of zif268-IR cells ± standard error of the mean in the (H) granular retrosplenial cortex C and (I) granular retrosplenial cortex AB. Zif268 cell density was the lower in primiparous hAPOEε4 rats compared to primiparous wildtype rats in the granular retrosplenial cortex. Average density of zif268-IR cells ± standard error of the mean in the (J) dorsal CA1, (K) dorsal DG, and (L) ventral DG. Zif268 cell density in the dorsal CA1 was lower in primiparous rats compared to nulliparous rats. Of all hippocampal subregions, zif2668 cell density was lowest in the dentate gyrus, with lower density in the dDG compared to vDG. * indicates p <0.05. CA – cornu ammonis, DG – dentate gyrus, zif268 – zinc finger-containing transcription factor 268, IR – immunoreactive, hAPOEε4 – humanized APOEε4

    Article Snippet: Tissue was incubated in primary antibody solution containing 1:500 goat anti-DCX (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and 1:1000 rabbit anti-zif268 (Egr-1 SC-189; Santa Cruz Biotechnology, Santa Cruz, CA, USA) in a dilution solution containing 1% normal donkey serum and 0.3% Triton-X in 0.1M PBS for 24 hours at 4°C.

    Techniques:

    (A-D) Heat maps generated based on correlation coefficients between density of zif268-IR cells in the different regions of interest and percentage of DCX/zif268 co-labelled cells in the dorsal and ventral dentate gyrus across groups. All negative (blue) and positive (red) correlations are included, and boxes with text indicate those with a coefficient | r | > 0.5. (E-H) Circular network maps and (I-L) geometric network maps were generated based on correlation coefficients between density of zif268-IR cells in the different regions of interest and percentage of DCX/zif268 co-labelled cells in the dorsal and ventral dentate gyrus across groups. Negative (blue) and positive (red) correlations with coefficient | r | > 0.5 were included. hAPOEε4 – humanized APOEε4

    Journal: bioRxiv

    Article Title: Parity and APOEε4 genotype contribute distinct changes to functional connectivity across the middle-aged brain

    doi: 10.1101/2025.03.01.640999

    Figure Lengend Snippet: (A-D) Heat maps generated based on correlation coefficients between density of zif268-IR cells in the different regions of interest and percentage of DCX/zif268 co-labelled cells in the dorsal and ventral dentate gyrus across groups. All negative (blue) and positive (red) correlations are included, and boxes with text indicate those with a coefficient | r | > 0.5. (E-H) Circular network maps and (I-L) geometric network maps were generated based on correlation coefficients between density of zif268-IR cells in the different regions of interest and percentage of DCX/zif268 co-labelled cells in the dorsal and ventral dentate gyrus across groups. Negative (blue) and positive (red) correlations with coefficient | r | > 0.5 were included. hAPOEε4 – humanized APOEε4

    Article Snippet: Tissue was incubated in primary antibody solution containing 1:500 goat anti-DCX (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and 1:1000 rabbit anti-zif268 (Egr-1 SC-189; Santa Cruz Biotechnology, Santa Cruz, CA, USA) in a dilution solution containing 1% normal donkey serum and 0.3% Triton-X in 0.1M PBS for 24 hours at 4°C.

    Techniques: Generated